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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene continues to be noticed in lots of tumors, which leads to chromosome instability, and so might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two further splice variants were described but in depth nucleotide sequence information will not be but available.

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Blocking peptides are peptides that bind specifically on the target antibody and block antibody binding. These peptide generally consists of the epitope identified by the antibody.

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This manual outlines further probable optimization of cellular and immunoassay parameters to make sure the best possible benefits are...

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This manual outlines more possible optimization of cellular and immunoassay parameters to be certain the very best results are...

Antibodies bound to the blocking peptide now not bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin more info E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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